Production of three phenylethanoids, tyrosol, hydroxytyrosol, and salidroside, using plant genes expressing in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Production of salidroside in metabolically engineered Escherichia coli
Salidroside (1) is the most important bioactive component of Rhodiola (also called as "Tibetan Ginseng"), which is a valuable medicinal herb exhibiting several adaptogenic properties. Due to the inefficiency of plant extraction and chemical synthesis, the supply of salidroside (1) is currently limited. Herein, we achieved unprecedented biosynthesis of salidroside (1) from glucose in a microorga...
متن کاملExpression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production
Salidroside, a plant secondary metabolite in Rhodiola, has been demonstrated to have several adaptogenic properties as a medicinal herb. Due to the limitation of plant source, microbial production of salidroside by expression of plant uridine diphosphate glycosyltransferase (UGT) is promising. However, glycoside production usually remains hampered by poor expression of plant UGTs in microorgani...
متن کاملExpressing genes in different Escherichia coli compartments.
Production of heterologous proteins or parts thereof in different extra-cytoplasmic compartments (in the periplasm, outer membrane or extracellularly) of Escherichia coli offers multiple applications, for example, in vaccine development, immobilised enzymes and bioremediation. Nowadays, not only surface display of short peptides, but also cell-surface anchoring or secretion of functional protei...
متن کاملEngineering of L-tyrosine oxidation in Escherichia coli and microbial production of hydroxytyrosol.
The hydroxylation of tyrosine is an important reaction in the biosynthesis of many natural products. The use of bacteria for this reaction has not been very successful due to either the over-oxidation to ortho-quinone when using tyrosinases from bacteria or plants, or the lack of the native cofactor, tetrahydrobiopterin (BH4), needed for the activity of tyrosine hydroxylases (TH). Here, we demo...
متن کاملThe Metabolism of Salidroside to Its Aglycone p-Tyrosol in Rats following the Administration of Salidroside
Salidroside is one of the major phenolic glycosides in Rhodiola, which has been reported to possess various biological activities. In the present study the in vivo deglycosylation metabolism of salidroside was investigated and its aglycone p-tyrosol but not the original salidroside was identified as the main form in rat tissues following the administration of salidroside. After the i.v. adminis...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-02042-2